An essential microscopy laboratory insturument...
The C2 confocal microscope system comprises a new generation of Nikon confocal instruments designed to be essential laboratory tools. Built on a reputation of incredible stability and operational simplicity coupled with superior optical technologies, the C2 with its host of functions and various analytical capabilities is the perfect tool for a new microscope, or as a new accessory to a Nikon imaging system.
Image Quality
Nikon's unprecedented optics and a time-proven, highly efficient optical design provide the brightest and sharpest images, at the longest working distances.
|
|
High efficiency scanning heads and detectors
The C2 fits all Nikon microscopes with the smallest scan head footprint on the market. The C2 employs high precision mirrors and optically ideal circular pinholes, enabling noiseless, high contrast and high quality confocal imaging. The spectral detector of the C2 enables high speed imaging using simultaneous 32-channel acquisition. Signal loss has been minimized while imaging of fluorescence spectra in real colors is possible by host of innovations for accurately correcting spectral data. |
| |
High-performance optics
CFI Apochromat S Series
These high NA objectives are ideal for confocal imaging with correction of chromatic aberrations over a wide wavelength range, from ultra violet to infrared. Transmission is increased through the use of Nikon’s exclusive Nano Crystal Coat technology.
|
CFI Apo 40xWI λS, NA1.25 (left)
CFI Apo LWD 40xWI λS, NA1.15 (middle)
CFI Apo 60x Oil λS, NA1.4 (right)
|
| |
CFI Apochromat TIRF Series
These objectives boast an unprecedented NA of 1.49 (using a standard coverslip and immersion oil), the highest resolution among Nikon objectives. The temperature correction ring corrects image quality affected by temperature change in the range of 23°C to 37°C.
 |
 |
CFI Apo TIRF 60x oil/1.49 (left)
|
CFI Apo TIRF 100x oil/1.49 (right)
|
|
| |
High definition diascopic DIC images
The C2 handles simultaneous 3-channel fluorescence or simultaneous 3-channel + diascopic DIC observation. High quality DIC images and fluorescence images can be superimposed to aid in image analysis such as locating fluorescence labels.
|
 |
 |
DIC image |
Overlay of DIC and fluorescence images |
High functionality
Nikon's renowned imaging software NIS-Elements enables intuitive operation of all Nikon hardware devices and peripheral. With a remarkably large number of analyzing functions for its class, the C2 supports your routine laboratory research activities.
|
| |
Multimode capability
All Nikon hardware is controlled by the same software as the top grade confocal system A1: one software package controls it all, and even at the same time! You can do confocal, widefield, TIRF, photoactivation acquisition, and processing, analysis, presentation — all in one software package.
|
 |
 |
Easy-to-recognize display for setting lasers, detectors, etc. |
Scanning parameter settings |
Large Image
Specimen: Genital tract of Drosophila melanogaster
Photo courtesy of: Director and Professor Masatoshi Yamamoto, Drosophila Genetic Resource Center, Kyoto Institute of Technology
|
| |
Unmixing
Numerous functions for analysis and unmixing of acquired spectrums are provided, while spectral profiles of general dyes and fluorescent proteins are preprogrammed.
|
 |
 |
The glomerulus, the main filter in mammalian kidneys, double stained with GFP and FITC. Both reagents have green fluorescence and emission maxima that are only a few nanometres apart. The picture shows the slide after spectral unmixing using NIS Elements' powerful unmixing algorithms, providing clearly distinguishable features. |
2D image of FluoCells (R) #4 from Molecular Probes (Invitrogen).
This is a section of a mouse intestine. The actin filaments are stained with Alexa Fluor 568 phalloidin and the nuclei are stained with SYTOX Green. |
| |
Multimode imaging system TIRF/Photo activation-C2
A TIRF laser illumination module and a photo activation module are integrated to enable both imaging of single molecules with extremely high S/N ratio and imaging of the fluorescence characteristic changes of photo-activated and photo-convertible fluorescent protein.
|
| |
Macro confocal microscope system AZ-C2
|
With high-definition widefield of view images larger than 1cm with unprecedentedly high S/N ratios, the AZ-C2 allows for imaging of whole-mount specimens, such as embryos, in a single shot. It offers a combination of low and high magnification objective lenses, optical zoom and a confocal scanning zoom function, enabling continuous imaging from macro to micro. Moreover, the AZ-C2 allows deep imaging of in-vivo whole specimens. |
|
|
 |
Plan Apochromat TIRF series
These objectives have the highest N.A. of all Nikon objectives. The 60x oil objective with the world’s first temperature correction ring can correct spherical aberration resulting from temperature-induced changes in the refractive index of the immersion oil from 23°C (room temperature) to 37°C (physiological temperature).
|
| |
| |
| |
