The C1si is equipped with two detectors—a traditional fluorescence one and one for spectral imaging—to manage a wide range of applications.
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One-shot Spectral Imaging
- Effective for observing live cell dynamics. (Simultaneous 32-channel spectral-image
acquisition)
- Allows spectral imaging that is gentle on live cells because a wide wavelength spectrum can
be captured with a single laser excitation.
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True Spectral Imaging
- Accurate fluorescence spectrum and true fluorescence colors can be obtained.
- Wavelength resolution independent of confocal pinhole diameters,* a leak-free laser beam
mechanism, sensitivity calibration between fluorescence detectors (using a NIST-compliant
light source),* calibration of optical wavelength transmittance characteristics,* and wavelength
calibration combine to achieve true spectral imaging.
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Spectral Imaging Focusing on Brightness
- Uncompromising optical design and signal processing perfectly trap fluorescence photons.
- Newly developed Diffraction Efficiency Enhancement System (DEES) technology* and highefficiency
fluorescence transmission technology* are used in the spectral system.
- Newly developed Dual Integration Signal Processing (DISP) technology* is used in the video
signal-processing circuitry to achieve brighter spectral imaging.
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Imaging of Unmixed Fluorescent Probes without Crosstalk
‧ Effective in the observation of expression-multiplexed specimens stained with CFP, GFP,
YFP, DsRed and similar reagents, as well as observation of localized protein molecules and
FRET observation. Separation of autofluorescence is also possible.
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