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RAMAN-11 is a next generation laser microscope observing an image of a sample with Raman scattering lights emitted from the molecules. Raman spectroscopy technique used for RAMAN-11 gives researchers the knowledge for the details of constituent elements of organelles or protein. By observing the Raman scattering lights emitted from the molecules of the living cells, distribution of the molecules can be imaged with the colors (wavelength) unique to those molecules without using the staining method. When a molecule is illuminated with a monochromatic laser beam, a portion of the incident light is scattered with different color. This scattered light is called "Raman scattering" for the name of the discoverer. The Raman scattered light tells us about the molecular is specific to the molecule, like a fingerprint. In addition, the Raman scattered light from a crystal gives the lattice condition of the crystal such as distortion, defects and impurity, and therefore can be utilized for crystal analysis.

Due to the weakness of Raman scattered light, conventional micro Raman system took a long time for one point measurement and scanned the sample point by point to take images. The scanning speed of this method is very slow. RAMAN-11 has noteworthy capable to accelerate the imaging speed. Nanophoton's original technology of a flying spot scanning technique contributes 100 times faster image acquisition than conventional systems.


Please Visit Nanophoton's Application page for detailed application descriptions


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Examples of observation:

Raman image of non-stained living cardiomyocytes. Raman peaks of lipid, protein, and nucleic acid are assigned to red, green and blue. Subcellular organelles and bio-molecules are observed.