Chamlide TC

Incubator system for various types of chambers

- Chamlide TC can be used on almost any microscope stage (Depending on the stage type, the Chamlide TC requires an incubator holder). The Chamlide TC was originally designed to fit stages with the universal mounting frame K standard stages or most XY-motorized stages.

- Chamlide TC can accept both various Chamlide magnetic chambers and commercial culture dishes using 5 types of adaptors.

- Chamlide TC includes special glass covers for disposable culture wares to maintain humidity and provide excellent transmission for imaging. (For Lab-Tek chambered coverglass, chambered slide, and 35mm culutre dish)

- The controller (CU-109) controls temperatures of the incubator main body, incubator cover, humidifier, and lens warmer, as well as adjust the flow rate of the mixed CO2 gas by using the flow meter (less than 100 ml/min).

- The FC-5 or FC-7 can provide optimum mixed gas to all incubator systems such as Chamlide TC, WP, and IC.

- Chamlide TC is available with in/out ports for perfusion or injection of liquids or drugs without having to open the incubator cover(using CM-B-PA, CM-B-PB).

- It is possible to control the temperature and gas concentration, program temperature and gas concentration gradients, and record the temperature and gas concentration over time using CCP ver 3.8 software or MetaMorph software.

Adaptors available

- TC adaptor for Chambered coverglass

: Nunc Lab-Tek™ II Chambered Coverglass (consumable, coverslip bottom)

- TC adaptor for 2-hole type Chamlide MB or Well-Slide/ Well-slip

: Chamlide MB(Chamlide magnetic chamber s), re-useable, coverslip bottom) , Well-Slide (re-useable, slide glass bottom), or Well-Slip (re-useable, coverslip bottom)

- TC adaptor for 35 mm dish type chamber or dish

: Commercial 35mm culture dishes (consumable) or 35mm dish type Chamlide magnetic chambers (re-useable)

- TC adaptor for Chamber Slide

: Nunc Lab-Tek™ II Chamber Slides (consumable,slide glass bottom)

ASI Piezo Z-stage:

Specifications

Warm, filtered air circulates within an acrylic enclosure from a heater unit which is mechanically isolated from the environmental chamber. Users may select temperatures within the range 32oC* to 42oC.

The specimen, being at the centre of the enclosure, is uniformly heated. It receives heat in X,Y and Z planes. Access to change specimens is provided through doors designed for both left and right handed researchers. Adjustments to the condenser and nosepiece are also made through these access ports whilst the regular controls of the microscope, e.g. focus and stage movement remain outside the enclosure. Accessories such as cameras, filter wheels, monochromators, micromanipulators, and multi-viewing heads can be accommodated.

The full enclosure incubation chamber provides a heated environment all around the specimen. The stage, nosepiece and a large part of the microscope frame are at physiological temperature. There is no need for supplementary objective and/or stage heaters.

Keeping the live cells under a humid 5% CO2 atmosphere maintains the pH of the growth medium. Humidity does not need to be ‘controlled’. It simply needs to be maintained close to the saturated vapour pressure of water at 37oC. Under these conditions evaporation is prevented and the cells remain stress free. The flow rate of the blanketing gas is low so there is little need for creating the CO2/air mixture in situ. It is more economical to use premixed 5% CO2 in air from a laboratory gas cylinder. In the case of Upright Microscopes, CO2 atmosphere control is possible when dipping objectives are used.

Maintaining living cells in the dark can further help to keep them stress free. In multi-photon microscopy the observed signal is very weak. The background noise from the detectors is significant so the signal to noise ratio is a matter of serious consideration. Current technology indicates that there is little that can be done to enhance the signal strength, but if the background noise is reduced by lowering the ambient light level, there is a significant increase in the signal to noise ratio. A low light level enclosure means that good experimental data can be obtained under red light conditions.

INU Series provides optimal culture environment for steady time-lapse, short/long term imaging with confocal, fluorescence, phase contrast, differential interference contrast and polarization applications. Major Applications: Short/long-term time-lapse and live cell imaging of signal transduction, formation, behavior, migration, propagation and differentiation during cell/tissue/organ culture under microscope in stem cell research, Cell/Molecular Biology, Biochemistry, Neuroscience, and in other research fields that require environment control.

THERMOPLATER is an optical clear glass heating device that provides consistent temperature control of specimen from directly beneath during microscopic observation. Wide product range supports almost all microscopes. Major Applications: Temperature control of specimens in the fields of IVF, stem cell research, Cell/Molecular Biology, Biochemistry, Electrical-Physiology and other research fields.

Properly conditioned gas is supplied during Live Cell Imaging Making stable 5% CO2+ 95% Air mixed gas supply from 100% CO2 possible. Major Applications: Stable CO2 mixed Gas supply during live cell culture in Stage Top Incubator INU Series.